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纸坊沟流域刺槐林土壤有机质演变特点及其累积机理研究
吕海波
学位类型学士
导师梁宗锁
2012-05
学位授予单位中国科学院研究生院
学位授予地点北京
关键词土壤有机质 水溶性有机质 刺槐林 Uv 吸收值
摘要

植被覆盖和土地利用方式的变化影响到土壤碳含量。大量研究发现农地转化为林地
后土壤碳贮量也随之发生变化。然而,森林生长序列中土壤碳的变化并不保持一致的增
长趋势。森林生态系统中土壤有机碳的动态变化机制的研究颇显重要。
研究通过对纸坊沟流域四种年限刺槐林的土壤有机质各组分的调查,在总结其特点
的基础上,分别设置野外培养试验和室内培养试验,旨在探索造成有机质变化的影响因
素。主要结论如下:
(1)对1975 年柠条林N75、1974 年耕地H74 和1978 年刺槐林C78 土壤剖面有机
碳、全N、土壤机械组成、碳酸钙、容重、pH 值等指标进行分析,发现各样地土壤有机
碳的积累与<0.02mm 粒径的土壤颗粒有较高的相关;土壤剖面各指标在不同深度存在异
常变化,<0.002mm 粘粒物质大幅度增加,表现出古土壤层的特征;1m 深度有机碳储量
C78>N75>H74,但差异主要表现在30cm 深度以上土层,特征层的存在可能导致H74 剖面
16.684g/m3 的有机碳评价误差,以及C78 剖面36.926g/m2 的误差。
(2)分析了类似立地条件下10 年、25 年、31 年、35 年四个刺槐林和一个对照农地
土壤有机碳SOC 各组分的变化。三深度平均SOC 含量、活性有机碳AOC 含量、水溶性有
机碳DOC 含量随刺槐林年限增加而增加;与农地相比,所有刺槐林样地AOC/SOC、惰性
有机碳ROC/SOC 呈高值表现,惰性有机碳LOC/SOC 值较低,DOC/水溶性有机氮DON 较低
而280nm 的UV 吸收值较高;随退耕年限的增加,25 年刺槐林表现出一个以深度分异为
代表的退耕特殊阶段。在刺槐林生长过程中,(i)AOC 和缓性有机碳ROC 都出现累积趋
势,刺槐林有助于土壤碳的储存;(ii)土壤碳的累积过程中有组分差异表现,DOC,
DOC/DON 以及UV 吸收值出现与SOC 不同的变化;(iii)各深度AOC 及SOC 并未表现出一
致的增长趋势,有深度分异。刺槐林生长发育过程中SOC 的积累有组分和深度的差异,
其相关机理需进一步研究。
(3)对1975 年柠条林N75、1974 年农地H74 和1978 年刺槐林C78 在100cm 深度
土壤剖面有机碳、全N、土壤机械组成、碳酸钙进行差异度分析和线性相关性分析,发
现退耕后两种林地SOC 及全N 含量虽然都有明显变化,但是C78 的差异主要表现在0-20cm 深度,而N75 则主要表现在20-100cm。相关度分析表明,H74 在20cm 以下SOC、
C/N 值都与0.002-0.02mm 粒径极显著相关,C78 在40cm 以下SOC 含量与<0.002mm 粒径
含量显著相关,C/N 值与<0.02mm 粒径由0-100cm 的负相关,变为正相关。结果证明柠
条林和刺槐林对SOC 和全N 的影响存在深度的差异,分别表现在深层和浅层。农地和刺
槐林土壤深层SOC 与细颗粒土壤显著相关,性质稳定。
(4)对C78样地均匀添加枯落物碎屑的土壤培养坑分3月、7月和11月进行一年期的
培养和观测,发现SOC各组分有季节性变化,20cm和40cm深度在7月SOC,活性有机碳POC
及总氮TN值最高,可溶性有机碳DOC和可溶性有机氮DON呈相反变化趋势;培养过程中各
指标出现深度分异,与20cm不同,在60cm深度,SOC随培养过程而递减,POC及全氮递增,
而DOC在7月出现高值。SOC在初始积累过程中先增后减,体现出自我调节。SOC各组分的
深度分异,反映出培养坑60 cm深度SOC来源出现多元化,枯落物腐解产物影响程度减弱。
(5)采集安塞纸坊沟31年刺槐林土样及林下混合枯落物,通过碱液吸收法测定
100%、20%和2%含水量条件下3深度土样20 cm,40 cm,60 cm、去除DOC土样(仅100%含
水量条件下)、三种处理枯落物混合土样(林下混合枯落物、刺槐枯落物和草本类枯落
物)培养过程中CO2的累计释放量。结果表明,100%和20%含水量条件下各深度土壤CO2
释放量为20 cm>60 cm>40 cm;20 cm土样去除DOC后CO2释放量明显减少,40 cm明显增加,
60 cm没有明显变化;混合枯落物土样在100%含水量条件下CO2释放量最高; 20%和2%含
水量条件下刺槐枯落物CO2释放量明显大于草类,而100%含水量条件下草类枯落物略大于
刺槐枯落物。研究证明土壤含水量对SOC组分含量和枯落物种类不同的土壤层呼吸强度
存在差异性影响,强降水对DOC的淋失可造成表层土壤呼吸的减弱。
(6)设置野外培养坑,将混合枯落物的土样和未混合枯落物的土样用致密的尼龙
布隔开,LDOM(难以自由迁移的那部分WEOM)的含量用两者同时培养后WEOM 含量的差
值来计算。野外培养两年并分段采样,在7 月份,样坑表层被吸附的WEOC(LDOC)和
WEOC 比率较其他时段低,并随样坑深度增加,比率升高;UV280nm 吸收值及LDON/WDON
较高,LDOC/WDOC 较低。 7 月20 cm LDOC/LDON 与WEOC/WEON 相当,但3 月和11 月前
者高于后者,说明7 月份LDOC/LDON 值与RDOC/RDON 没有明显差异,但在其他时间前者
呈高值表现。研究证明LDOM 有季节性和深度分异,当比较WEOM 和DOM 的研究结果时,
应当注意到这个问题。
关键词:土壤有机质;水溶性有机质;刺槐林;UV 吸收值

其他摘要

Soils have the potential for C release or sequestration,can be affected by vegetation
cover and land management. Many studies found the transformation of cultivated soil to
forest ecosystem can change soil carbon sequestering. However, soil carbon stock change in
forest chronosequence is likely to appear different results. Hence, accurate estimate on the
mechanism of SOC dynamics in forest chronosequences is necessary. The study investigated
the variation patterns of organic matter of four selected Robinia pseudoacacia forests aged 10,
25, 31, 35 years, and explore the rules. On basias of the rules, we made field and labortary
incubational experimentes to explore the effected factors. The main conclusions were showed
as follows:
(1)The study gives a anlysis on indexes such as SOC、Tatol nitrogen、particle size、
CaCO3、CECand pH,etc,in the soil profile of Caragana Korshinskii Kom planted in 1975 、
plowland since 1974 and Robinia pseudoacadia planted in 1978. Soil organic carbon
accumulation in each site has a high correlation with the percent of <0.02mm diameter; some
factors in soil profiles exist unnormal changes in different depths, the <0.002mm clay matrial
has a substantial increase, represents the charactors of illuvial horizon; In the depth of
100cm,it is C78>N75>H74 on SOC content, however, the main difference was reflected in the
upper 30cm depth. The presence of ancient soil lead to 16.684g/m3 error on H74 SOC
assessment, as well as 36.926g/m2 Error on C78 SOC assessment.
(2)We investigated the variation patterns of organic carbon of four selected Robinia
pseudoacacia forests aged 10, 25, 31, 35 years, and a contrastive tillage site in similar
topography condition. On average of 20cm, 40cm, 60cm depth, SOC, active organic carbon
(AOC), DOC gradually increase with the forest growth. Compared with tillage sites,
AOC/SOC ratio, ROC (resistant organic carbon)/SOC ratio show a higher value, and LOC
(slow organic carbon)/SOC ratio shows a lower value. DOC/DON is lower and UV absorption
at 280 nm shows a higher value in all forest sites. During forest restoration, 25-year-old
Robinia pseudoacacia forest shows a special phase in four forest sites represented by depth
distribution. During forest restoration (i) not only AOC, but ROC take an accumulation
process, Robinia pseudoacacia forest restoration contributes to SOC accumulation. (ii) DOC,
DOC/DON, UV-absorbance appear changes but not obviously accordant to SOC, suggesting
different changes during SOC accumulation. (iii) SOC, AOC don’t show a persistent increase  in each depth of soil profile and which indicate a depth distribution. Our study found SOC
had compounds-distribution and depth-distribution, but the relative machisms need to be
explore.
(3)Analyzed the quantity of SOC, total nitrogen, particle size, CaCO3 for the diversity
analysis and the correlation ratio in 100cm soil profiles respectly in Caragana Korshinskii
Kom forest planted in 1975(N75) 、tillage since 1974(H74) and Robinia pseudoacadia forest
planted in 1978(N78) . The results suggest that compared with tillage site, though SOC and
total of nitrogen in two restored forest sites show obvious changes, the distinct diversity in
C78 appears mainly in the depth of 0-20cm, and the same state appears at 20-100cm for N75.
At 20-100cm in H74 site, SOC, C/N show remarkable correlations with percent of
0.002-0.02mm particle size. Below 40cm in C78 site, SOC are remarkable correlation with
<0.002mm particle size, the correlation rate between C/N and <0.02mm changes from minus
value for 0-100cm depth to positive value for 40-100cm. Our study indicate that after forest
restoration, changes of SOC and total of nitrogen mainly represent a obvious diversity at 40
cm and 60 cm soil layer for Caragana Korshinskii Kom forest, but diversity appearsat 20 cm
for Robinia pseudoacadia forest. SOC in deep soil layer of tillage and Robinia pseudoacadia
forest is closely adsorbed by fine particle size soil, with a steady quality.
(4)We have observed the dynamics of SOC and it’s relative factors of three depths
(20cm,40cm,60cm) incubation holes, which were backfilled soils mixed with litter rags in
advance, in C78 Robinia pseudoacacia forest, and took samples in March, July and November,
2010. The results represent reasonal changes, at 20cm and 40cm, SOC, POC, TN in July are
highest, but decrease in November, and reverse states represent for DOC. Different with those
at 20 cm, SOC at 60 cm decreases gradually in the process of incubation and TN and POC
have increase trend, DOC in July appears a high value. During C accumulation, SOC
increases but decrease later, represents self-modulation function. DOC has different sources
for deeper soil layer with those at 20 cm, SOC suggests complex sources at 60 cm, the
production of litters decomposition have limited effect.
(5)Took soil and mixed litter samples in 31 year old Robinia Pseudoacacia forest and
determined the accumulative CO2-release of soil samples and DOC-removal soil samples
(100% water content only) at three soil layers (20 cm,40 cm,60 cm), three controlled
litter-mixed soil samples (mixed by complex litters, included Robinia Pseudoacacia litters
and herbage litters), in laboratory under the different soil moisture levels of 100%, 20%, 2%
water content respectively. The results showed that the CO2-release levels in different depths
were suggested by 20 cm>60 cm>40 cm in controlled 100% and 20% water content; In the  condition of 100% water content, when DOC were removed, the accumulative CO2-release
quantity of 20 cm soil samples decreased, but obviously increase for samples at 40 cm and
had no marked change at 60 cm; The accumulative CO2-release of complex litter-mixed soil
samples had highest value in the condition of 100% water content, those of simply Robinia
Pseudoacacia litter-mixed samples was obvious higher than that of herbage litter-mixed
samples in 20% and 2%water content, but slightly lower in 100% water content. The
asynchronous effect of water content levels is suggested on the respiratory intensity of the soil
layers with different SOC compositions and litter kinds, heavy precipitation removes DOC
from soil subsurface layers, can weaken the soil respiratory intensity.
(6)In two-year incubation experiment, litter-mixture soil (outside bags) and no
litter-mixture soil (inside bags) were insulated by nylon cloth in incubation holes, changes in
the LDOM portion of the soil were then monitored by comparing the WEOM levels outside
the bags with inside the bags., In July, the ratio of locked WEOC (LDOC) to WEOC outside
bags (LDOC/WEOC) was low in the topsoil, and increased gradually with depth while the
locked WEON (LDON) to WEON ratio outside bags (LDON/WEON) suggested no obvious
rule. At 20 cm In July, LDOC/WEOC were lower and LDON/WEON were higher than those
in March and November, solutions of the soil samples had highest UV-absorbance at 280 nm,
The LDOC:LDON ratio at 20 cm was comparable in July with, but higher in March and
November than WEOC:WEON ratio of samples outside bags, which implied that
LDOC:LDON has little difference compared to that of RDOM in July, but represents higher
value at other times of the year. The study proves that LDOM has seasonal variability and
depth distribution, which brings a difficult to distinguish the difference between WEOM and
DOM (RDOM).
Key Wards:Keywords: soil organic matter; water extractable organic matter;
Robinia pseudoacacia forests; UV-absorbance

语种中文
文献类型学位论文
条目标识符sbir.nwafu.edu.cn/handle/361005/8939
专题水保所知识产出(1956---)
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吕海波. 纸坊沟流域刺槐林土壤有机质演变特点及其累积机理研究[D]. 北京. 中国科学院研究生院,2012.
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