KMS Institute of soil and water conservation Chinese Academy of Sciences
| Rhizobium inoculation enhances copper tolerance by affecting copper uptake and regulating the ascorbate-glutathione cycle and phytochelatin biosynthesis-related gene expression in Medicago sativa seedlings | |
| Juan Chen1,2; Yu-Qing Liu1; Xiao-Wu Yan4; Ge-Hong Wei4; Jian-Hua Zhang2,3; Lin-Chuan Fang1; Fang, LC (reprint author), Northwest A&F Univ, State Key Lab Soil Eros & Dryland Fanning Loess P, Yangling 712100, Shaanxi, Peoples R China. | |
| 文章类型 | Article |
| 2018 | |
| 发表期刊 | ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
 |
| ISSN | 0147-6513 |
| 通讯作者邮箱 | flinc629@hotmail.com |
| 卷号 | 162页码:312-323 |
| 摘要 | Despite numerous reports that legume-rhizobium symbiosis alleviates Cu stress in plants, the possible roles of legume-rhizobium symbiosis and the regulatory mechanisms in counteracting Cu toxicity remain unclear. Here, Sinorhizobium meliloti CCNWSX0020 was used for analyzing the effects of rhizobium inoculation on plant growth in Medicago saliva seedlings under Cu stress. Our results showed that rhizobium inoculation alleviated Cu-induced growth inhibition, and increased nitrogen concentration in M. sativa seedlings. Moreover, the total amount of Cu uptake in inoculated plants was significantly increased compared with non-inoculated plants, and the increase in the roots was much higher than that in the shoots, thus decreasing the transfer coefficient and promoting Cu phytostabilization. Cu stress induced lipid peroxidation and reactive oxygen species production, but rhizobium inoculation reduced these components' accumulation through altering antioxidant enzyme activities and regulating ascorbate-glutathione cycles. Furthermore, legume-rhizobium symbiosis regulated the gene expression involved in antioxidant responses, phytochelatin (PC) biosynthesis, and metallothionein bio-synthesis in M. sativa seedlings under Cu stress. Our results demonstrate that rhizobium inoculation enhanced Cu tolerance by affecting Cu uptake, regulating antioxidant enzyme activities and the ascorbate-glutathione cycle, and influencing PC biosynthesis-related gene expression in M. sativa. The results provide an efficient strategy for phytoremediation of Cu-contaminated soils. |
| 关键词 | Antioxidant Enzymes Ascorbate-glutathione Cycle Copper Stress Legume-rhizobium Phytoremediation |
| 学科领域 | Environmental Sciences & Ecology ; Toxicology |
| DOI | 10.1016/j.ecoenv.2018.07.001 |
| 收录类别 | SCI |
| 出版地 | 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA |
| 语种 | 英语 |
| WOS记录号 | WOS:000440879500037 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| 项目资助者 | National Key Research and Development Program of China [2016YFD0800706] ; National Key Research and Development Program of China [2016YFD0800706] ; National Natural Science Foundation of China (NSFC) [31501822, 41571314] ; National Natural Science Foundation of China (NSFC) [31501822, 41571314] |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | sbir.nwafu.edu.cn/handle/361005/8180 |
| 专题 | 水保所科研产出--SCI_2018--SCI |
| 通讯作者 | Fang, LC (reprint author), Northwest A&F Univ, State Key Lab Soil Eros & Dryland Fanning Loess P, Yangling 712100, Shaanxi, Peoples R China. |
| 作者单位 | 1.Northwest A&F Univ, State Key Lab Soil Eros & Dryland Fanning Loess P, Yangling 712100, Shaanxi, Peoples R China 2.Chinese Univ Hong Kong, Sch Life Sci, Hong Kong, Hong Kong, Peoples R China 3.Chinese Univ Hong Kong, State Key Lab Agrobiotechnol, Hong Kong, Hong Kong, Peoples R China 4.Northwest A&F Univ, Sch Life Sci, Yangling 712100, Shaanxi, Peoples R China 5.Hong Kong Baptist Univ, Dept Biol, Hong Kong, Hong Kong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Juan Chen,Yu-Qing Liu,Xiao-Wu Yan,et al. Rhizobium inoculation enhances copper tolerance by affecting copper uptake and regulating the ascorbate-glutathione cycle and phytochelatin biosynthesis-related gene expression in Medicago sativa seedlings[J]. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,2018,162:312-323. |
| APA | Juan Chen.,Yu-Qing Liu.,Xiao-Wu Yan.,Ge-Hong Wei.,Jian-Hua Zhang.,...&Fang, LC .(2018).Rhizobium inoculation enhances copper tolerance by affecting copper uptake and regulating the ascorbate-glutathione cycle and phytochelatin biosynthesis-related gene expression in Medicago sativa seedlings.ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,162,312-323. |
| MLA | Juan Chen,et al."Rhizobium inoculation enhances copper tolerance by affecting copper uptake and regulating the ascorbate-glutathione cycle and phytochelatin biosynthesis-related gene expression in Medicago sativa seedlings".ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 162(2018):312-323. |
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