水保所知识产出(1956---)知识库

 采用亚硝酸钠(NaNO 2 )在酸性条件下与还原剂还原型谷胱甘肽(GSH)反应生成 S － 亚硝基谷胱甘肽
(GSNO)，GSNO 能够释放出 NO，合成不同浓度的 GSNO(0、50、125、250 μmol·L
－1 和 350 μmol·L －1 )溶液处理受
CdCl 2 胁迫的玉米(Zea mays L． )幼苗，以未用 CdCl 2 处理的玉米幼苗作为对照(Control)，探究 GSNO 对玉米幼苗 Cd
胁迫的缓解作用及其抗氧化机制。结果表明:GSNO 处理使 Cd 胁迫下玉米幼苗的株高、茎粗和叶面积与对照组相比
显著增加，其中250 μmol·L
－1 浓度的 GSNO 处理效果最佳;在350 μmol·L －1 GSNO 处理下玉米幼苗各类光合色素含
量在胁迫组均达到最大值，有效缓解了叶片黄化;GSNO 处理(最适浓度在 125 ～250 μmol·L
－1 之间)也显著增强了
Cd 胁迫下玉米幼苗的抗氧化酶活性，如超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、抗坏血酸过氧化物酶(APX)和
过氧化物酶(POD)的活性，相应地降低了过氧化氢(H 2 O 2 )和超氧阴离子(O 2 · )含量的积累;GSNO 还可以显著提高
玉米幼苗螯合重金属离子的能力，其中 250 μmol·L
－1 浓度的 GSNO 处理效果最佳。

 Gaseous signaling molecule nitric oxide (NO) is proven in many plants to be able to alleviate cadmi-
um (Cd) stress． Under acidic conditions，glutathione (GSH) can react with sodium nitrite (NaNO 2 ) to generate S-
nitroso-glutathione (GSNO)，which can release NO． The present work was to study whether this type of NO could
help maize seedlings (Zea mays L． ) to resist Cd stress． Different concentrations of GSNO (50，125，250 μmol·
L
－1 ，and 350 μmol·L －1 ) were applied to maize seedlings that had been subjected to 80 mg·L －1
CdCl 2 for 7 d．
The results showed that GSNO could significantly improve maize seedlings plant height，stem diameter，and leaf area，
especially at the 250 μmol·L
－1
GSNO concentration; The addition of 350 μmol·L
－1
GSNO couldmaintain photo-
synthetic pigment maximization，effectively alleviate leaf yellowing; The benificial effects of GSNO (optimum concen-
tration was in the range of 150 ～250 μmol·L
－1 ) on Cd-stressed maize might be related to the enhanced antioxidant
enzyme activity，such as superoxide dismutase (SOD)，catalase (CAT)，ascorbate peroxidase (APX) and peroxi-
dase (POD)，and decreased levels of H 2 O 2 and O 2 · ; Furthermore，GSNO greatly enhanced the capacity of maize
seedlings to chelate heavy metal ions which optimal GSNO concentration was 250 μmol·L
－1 ．