Molecular cloning and characterisation of two enzymes involved in the rosmarinic acid biosynthesis pathway of Prunella vulgaris L.

doi:10.1007/s11240-016-1117-z

ISWC OpenIR > 水保所科研产出--SCI > 2017--SCI

	Molecular cloning and characterisation of two enzymes involved in the rosmarinic acid biosynthesis pathway of Prunella vulgaris L.
	Ru, Mei 1,2; Wang, Kunru 3; Bai, Zhenqing 3; Peng, Liang 4; He, Shaoxuan 5; Pei, Tianlin 3; Jia, Yanyan 3; Li, Hongyan 3; Liang, Zongsuo 1,2; Liang, ZS (reprint author), Chinese Acad Sci, Inst Soil & Water Conservat, Yangling 712100, Peoples R China.; Liang, ZS (reprint author), Chinese Acad Sci, Minist Water Resources, Yangling 712100, Peoples R China.; Liang, ZS (reprint author), Northwest A&F Univ, Coll Life Sci, Yangling, Peoples R China.
文章类型	Article
	2017
发表期刊	PLANT CELL TISSUE AND ORGAN CULTURE
ISSN	0167-6857
通讯作者邮箱	liangzs@ms.iswc.ac.cn
卷号	128 期号:2 页码:381-390
摘要	Prunella vulgaris, a widely-used medicinal perennial herb, contains various active compounds and has multifaceted medicinal activities. As the index component in P. vulgaris, rosmarinic acid (RA) is a typical phenolic acid, with significant anti-inflammatory, anti-oxidant, anti-tumour, anti-viral, and anti-microbial properties. To better understand the RA biosynthetic pathway in P. vulgaris, we isolated and cloned the cDNA sequences of putative RA synthase (PvRAS) and cytochrome P450 monooxygenase (PvCYP98A101), two important enzymes catalysing RA biosynthesis. Sequence analysis revealed that PvRAS contained an open reading frame (ORF) of 1305 bp encoding a 435-amino-acid residue belonging to the BAHD acyltransferase family, and PvCYP98A101 contained an ORF of 1530 bp encoding a 510-amino-acid residue, as a member of the CYP98A family. The deduced PvRAS and PvCYP98A101 amino acid sequences shared high similarity with other putative/known RASs and CYPs, respectively. Quantitative real-time PCR analysis showed that constitutive expression of PvRAS and PvCYP98A101 was much higher in roots than in leaves, stems, or spikes. Further analysis indicated that PvRAS was localised in the cytosol and nucleus, whilst PvCYP98A101 existed as a membrane protein in the endoplasmic reticulum. These results highlight the RA biosynthesis pathway in P. vulgaris and, provide useful information to engineer natural products.
关键词	Prunella Vulgaris Rosmarinic Acid Rosmarinic Acid Synthase Cytochrome P450 Monooxygenase Subcellular Localisation Expression Pattern
学科领域	Biotechnology & Applied Microbiology ; Plant Sciences
DOI	10.1007/s11240-016-1117-z
URL	查看原文
收录类别	SCI
出版地	DORDRECHT
语种	英语
WOS记录号	WOS:000393748500013
出版者	SPRINGER
项目资助者	Twelfth Five-Year Plan for Science & Technology Support of China [2015BAC01B03]; Doctoral Fund of Ministry of Education of China [20110204110028]; National Natural Science Foundation of China [81373908] ; Twelfth Five-Year Plan for Science & Technology Support of China [2015BAC01B03]; Doctoral Fund of Ministry of Education of China [20110204110028]; National Natural Science Foundation of China [81373908]
引用统计
文献类型	期刊论文
条目标识符	sbir.nwafu.edu.cn/handle/361005/8040
专题	水保所科研产出--SCI_2017--SCI
通讯作者	Liang, ZS (reprint author), Chinese Acad Sci, Inst Soil & Water Conservat, Yangling 712100, Peoples R China.; Liang, ZS (reprint author), Chinese Acad Sci, Minist Water Resources, Yangling 712100, Peoples R China.; Liang, ZS (reprint author), Northwest A&F Univ, Coll Life Sci, Yangling, Peoples R China.
作者单位	1.Chinese Acad Sci, Inst Soil & Water Conservat, Yangling 712100, Peoples R China 2.Chinese Acad Sci, Minist Water Resources, Yangling 712100, Peoples R China 3.Northwest A&F Univ, Coll Life Sci, Yangling, Peoples R China 4.Shaanxi Univ Chinese Med, Coll Pharm, Xian, Peoples R China 5.Environm Protect Agcy, Environm Monitoring Stn, Chongqing, Peoples R China
推荐引用方式 GB/T 7714	Ru, Mei,Wang, Kunru,Bai, Zhenqing,et al. Molecular cloning and characterisation of two enzymes involved in the rosmarinic acid biosynthesis pathway of Prunella vulgaris L.[J]. PLANT CELL TISSUE AND ORGAN CULTURE,2017,128(2):381-390.
APA	Ru, Mei.,Wang, Kunru.,Bai, Zhenqing.,Peng, Liang.,He, Shaoxuan.,...&Liang, ZS .(2017).Molecular cloning and characterisation of two enzymes involved in the rosmarinic acid biosynthesis pathway of Prunella vulgaris L..PLANT CELL TISSUE AND ORGAN CULTURE,128(2),381-390.
MLA	Ru, Mei,et al."Molecular cloning and characterisation of two enzymes involved in the rosmarinic acid biosynthesis pathway of Prunella vulgaris L.".PLANT CELL TISSUE AND ORGAN CULTURE 128.2(2017):381-390.