Isolation and characterization of LcSAP, a Leymus chinensis gene which enhances the salinity tolerance of Saccharomyces cerevisiae

doi:10.1007/s11033-016-4091-y

ISWC OpenIR > 水保所科研产出--SCI > 2017--SCI

	Isolation and characterization of LcSAP, a Leymus chinensis gene which enhances the salinity tolerance of Saccharomyces cerevisiae
	Liu, Jingying 1,2; Yang, Xiangna 2; Yang, Xizhe 2; Xu, Mingyue 2; Liu, Jie 2; Xue, Mengmeng 2; Ma, Pengda 2; Ma, PD (reprint author), Northwest A&F Univ, Coll Life Sci, Yangling 712100, Peoples R China.
文章类型	Article
	2017
发表期刊	MOLECULAR BIOLOGY REPORTS
ISSN	0301-4851
通讯作者邮箱	mapengda@163.com
卷号	44 期号:1 页码:5-9
摘要	A number of members of the SAP ("stress-associated protein") gene family have been implicated in the plant stress response. Here, a SAP gene has been isolated using PCR RACE from the perennial grass Leymus chinensis, a species which has reputation for ecological adaptability. The 17.6 kDa LcSAP product comprised 161 residues, including both an A20 domain and an AN1 domain, a feature of type I SAPs. Using a semi-quantitative RT-PCR assay to profile its transcription, it was shown that LcSAP was more strongly transcribed in the leaf than in the root under control conditions. The level of LcSAP transcription began to rise 6 h after the plant's exposure to 400 mM NaCl, and the abundance of transcript remained stable for at least 24 h. Exposing the plant to 100 mM Na2CO3 also induced LcSAP transcription, but the abundance of SAP transcript faded after 6 h. When LcSAP was introduced into yeast cells, the transgenic cells grew better than wild type ones when the medium contained 1.4 M NaCl. The ability of LcSAP to respond to salinity stress in yeast suggests that it also makes a contribution to the stress tolerance shown by L. chinensis.
关键词	Stress Associated Protein Salinity Leymus Chinensis Saccharomyces Cerevisiae
学科领域	Biochemistry & Molecular Biology
DOI	10.1007/s11033-016-4091-y
URL	查看原文
收录类别	SCI
出版地	DORDRECHT
语种	英语
WOS记录号	WOS:000394349600002
出版者	SPRINGER
项目资助者	Chinese Universities Scientific Fund [2452016006, 2014YB044]; Specialized Research Fund for the Doctoral Program of Higher Education [20130204120018, 20130204120038]; Natural Science Basic Research Plan in Shaanxi Province of China [2013JQ3006]; National Natural Science Foundation of China [31401839, 31670295]; Institute of Soil and Water Conservation, NWAFU [A314021402-1522] ; Chinese Universities Scientific Fund [2452016006, 2014YB044]; Specialized Research Fund for the Doctoral Program of Higher Education [20130204120018, 20130204120038]; Natural Science Basic Research Plan in Shaanxi Province of China [2013JQ3006]; National Natural Science Foundation of China [31401839, 31670295]; Institute of Soil and Water Conservation, NWAFU [A314021402-1522]
引用统计
文献类型	期刊论文
条目标识符	sbir.nwafu.edu.cn/handle/361005/8038
专题	水保所科研产出--SCI_2017--SCI
通讯作者	Ma, PD (reprint author), Northwest A&F Univ, Coll Life Sci, Yangling 712100, Peoples R China.
作者单位	1.Northwest A&F Univ, State Key Lab Soil Eros & Dryland Farming Loess P, Yangling 712100, Peoples R China 2.Northwest A&F Univ, Coll Life Sci, Yangling 712100, Peoples R China
推荐引用方式 GB/T 7714	Liu, Jingying,Yang, Xiangna,Yang, Xizhe,et al. Isolation and characterization of LcSAP, a Leymus chinensis gene which enhances the salinity tolerance of Saccharomyces cerevisiae[J]. MOLECULAR BIOLOGY REPORTS,2017,44(1):5-9.
APA	Liu, Jingying.,Yang, Xiangna.,Yang, Xizhe.,Xu, Mingyue.,Liu, Jie.,...&Ma, PD .(2017).Isolation and characterization of LcSAP, a Leymus chinensis gene which enhances the salinity tolerance of Saccharomyces cerevisiae.MOLECULAR BIOLOGY REPORTS,44(1),5-9.
MLA	Liu, Jingying,et al."Isolation and characterization of LcSAP, a Leymus chinensis gene which enhances the salinity tolerance of Saccharomyces cerevisiae".MOLECULAR BIOLOGY REPORTS 44.1(2017):5-9.