A tyrosine aminotransferase involved in rosmarinic acid biosynthesis in Prunella vulgaris L

doi:10.1038/s41598-017-05290-4

ISWC OpenIR > 水保所科研产出--SCI > 2017--SCI

	A tyrosine aminotransferase involved in rosmarinic acid biosynthesis in Prunella vulgaris L
	Ru, Mei 1,2; Wang, Kunru 1,2; Bai, Zhenqing 1,2; Peng, Liang 3; He, Shaoxuan 4; Wang, Yong 1,2; Liang, Zongsuo 1,2; Liang, ZS (reprint author), Chinese Acad Sci, Inst Soil & Water Conservat, Yangling 712100, Peoples R China.; Liang, ZS (reprint author), Minist Water Resources, Yangling 712100, Peoples R China.; Liang, ZS (reprint author), Zhejiang Sci Tech Univ, Coll Life Sci, Hangzhou 310000, Zhejiang, Peoples R China.
文章类型	Article
	2017
发表期刊	SCIENTIFIC REPORTS
ISSN	2045-2322
通讯作者邮箱	liangzs@ms.iswc.ac.cn
卷号	7
摘要	Rosmarinic acid (RA) and its derivants are medicinal compounds that comprise the active components of several therapeutics. We isolated and characterised a tyrosine aminotransferase of Prunella vulgaris (PvTAT). Deduced PvTAT was markedly homologous to other known/putative plant TATs. Cytoplasmic localisation of PvTAT was observed in tobacco protoplasts. Recombinantly expressed and purified PvTAT had substrates preference for L-tyrosine and phenylpyruvate, with apparent K-m of 0.40 and 0.48 mM, and favoured the conversion of tyrosine to 4-hydroxyphenylpyruvate. In vivo activity was confirmed by functional restoration of the Escherichia coli tyrosine auxotrophic mutant DL39. Agrobacterium rhizogenes-mediated antisense/sense expression of PvTAT in hairy roots was used to evaluate the contribution of PvTAT to RA synthesis. PvTAT were reduced by 46-95% and RA were decreased by 36-91% with low catalytic activity in antisense transgenic hairy root lines; furthermore, PvTAT were increased 0.77-2.6- fold with increased 1.3-1.8-fold RA and strong catalytic activity in sense transgenic hairy root lines compared with wild-type counterparts. The comprehensive physiological and catalytic evidence fills in the gap in RA-producing plants which didn't provide evidence for TAT expression and catalytic activities in vitro and in vivo. That also highlights RA biosynthesis pathway in P. vulgaris and provides useful information to engineer natural products.
学科领域	Science & Technology - Other Topics
DOI	10.1038/s41598-017-05290-4
URL	查看原文
收录类别	SCI
出版地	LONDON
语种	英语
WOS记录号	WOS:000404970900054
出版者	NATURE PUBLISHING GROUP
项目资助者	Twelfth Five-Year Plan for Science & Technology Support of China [2015BAC01B03]; National Natural Science Foundation of China [81373908] ; Twelfth Five-Year Plan for Science & Technology Support of China [2015BAC01B03]; National Natural Science Foundation of China [81373908]
引用统计	被引频次：9[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	sbir.nwafu.edu.cn/handle/361005/7961
专题	水保所科研产出--SCI_2017--SCI
通讯作者	Liang, ZS (reprint author), Chinese Acad Sci, Inst Soil & Water Conservat, Yangling 712100, Peoples R China.; Liang, ZS (reprint author), Minist Water Resources, Yangling 712100, Peoples R China.; Liang, ZS (reprint author), Zhejiang Sci Tech Univ, Coll Life Sci, Hangzhou 310000, Zhejiang, Peoples R China.
作者单位	1.Chinese Acad Sci, Inst Soil & Water Conservat, Yangling 712100, Peoples R China 2.Minist Water Resources, Yangling 712100, Peoples R China 3.Shannxi Univ Chinese Med, Coll Pharm, Xian 710000, Peoples R China 4.Environm Protect Agcy, Ecol Environm Monitoring Stn, Dazu 402360, Peoples R China 5.Zhejiang Sci Tech Univ, Coll Life Sci, Hangzhou 310000, Zhejiang, Peoples R China
推荐引用方式 GB/T 7714	Ru, Mei,Wang, Kunru,Bai, Zhenqing,et al. A tyrosine aminotransferase involved in rosmarinic acid biosynthesis in Prunella vulgaris L[J]. SCIENTIFIC REPORTS,2017,7.
APA	Ru, Mei.,Wang, Kunru.,Bai, Zhenqing.,Peng, Liang.,He, Shaoxuan.,...&Liang, ZS .(2017).A tyrosine aminotransferase involved in rosmarinic acid biosynthesis in Prunella vulgaris L.SCIENTIFIC REPORTS,7.
MLA	Ru, Mei,et al."A tyrosine aminotransferase involved in rosmarinic acid biosynthesis in Prunella vulgaris L".SCIENTIFIC REPORTS 7(2017).