Identification, cloning and characterization of R2R3-MYB gene family in canola (Brassica napus L.) identify a novel member modulating ROS accumulation and hypersensitive-like cell death

doi:10.1093/dnares/dsv040

ISWC OpenIR > 水保所科研产出--SCI > 2016--SCI

	Identification, cloning and characterization of R2R3-MYB gene family in canola (Brassica napus L.) identify a novel member modulating ROS accumulation and hypersensitive-like cell death
	Bisi Chen; Fangfang Niu; Wu-Zhen Liu; Bo Yang; Jingxiao Zhang; Jieyu Ma; Hao Cheng; Feng Han; Yuan-Qing Jiang
文章类型	Article
	2016
发表期刊	DNA Research
通讯作者邮箱	jiangyq@nwafu.edu.cn
卷号	23 期号:2 页码:101-14
摘要	The R2R3-MYB proteins comprise one of the largest families of transcription factors in plants. Although genome-wide analysis of this family has been carried out in some plant species, little is known about R2R3-MYB genes in canola (Brassica napus L.). In this study, we have identified 76 R2R3-MYB genes in the canola genome through mining of expressed sequence tags (ESTs). The cDNA sequences of 44 MYB genes were successfully cloned. The transcriptional activities of BnaMYB proteins encoded by these genes were assayed in yeast. The subcellular localizations of representative R2R3-MYB proteins were investigated through GFP fusion. Besides, the transcript abundance level analysis during abiotic conditions and ABA treatment identified a group of R2R3- MYB genes that responded to one or more treatments. Furthermore, we identified a previously functionally unknown MYB gene-BnaMYB78, which modulates reactive oxygen species (ROS)- dependent cell death in Nicotiana benthamiana, through regulating the transcription of a few ROS- and defence-related genes. Taken together, this study has provided a solid foundation for understanding the roles and regulatory mechanism of canola R2R3-MYB genes.
关键词	Abiotic Stress Brassica Napus Myb Ros Cell Death
DOI	10.1093/dnares/dsv040
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000375088800003
引用统计	被引频次：16[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	sbir.nwafu.edu.cn/handle/361005/7815
专题	水保所科研产出--SCI_2016--SCI
通讯作者	Yuan-Qing Jiang
作者单位	State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau and College of Life Sciences, Northwest A & F University, Yangling, Shaanxi 712100, China
推荐引用方式 GB/T 7714	Bisi Chen,Fangfang Niu,Wu-Zhen Liu,et al. Identification, cloning and characterization of R2R3-MYB gene family in canola (Brassica napus L.) identify a novel member modulating ROS accumulation and hypersensitive-like cell death[J]. DNA Research,2016,23(2):101-14.
APA	Bisi Chen.,Fangfang Niu.,Wu-Zhen Liu.,Bo Yang.,Jingxiao Zhang.,...&Yuan-Qing Jiang.(2016).Identification, cloning and characterization of R2R3-MYB gene family in canola (Brassica napus L.) identify a novel member modulating ROS accumulation and hypersensitive-like cell death.DNA Research,23(2),101-14.
MLA	Bisi Chen,et al."Identification, cloning and characterization of R2R3-MYB gene family in canola (Brassica napus L.) identify a novel member modulating ROS accumulation and hypersensitive-like cell death".DNA Research 23.2(2016):101-14.

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