To detect the expression of PIP2-5 in maize root systems under water deficit,semi-quantitative PCR was used. The reference gene was tubulin. Maize seedlings were planted under the condition of hydroponics culture in growth chamber. Water deficit was simulated by using PEG-6000. The results showed that PIP2-5 was up-regulated under water deficit,which supported the theory that the contribution of cell-to-cell pass-way for root systems uptake maybe increased under water deficit.
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