水保所知识产出(1956---)知识库

Fungal richness and community composition are known to be positively associated with soil multifunctionality.
However, the contributions of bacterial and fungal communities to the multiple soil functions of alpine meadow
ecosystems have not been widely examined. Here, we surveyed the soil in Qinghai-Tibetan alpine meadows and
classified the extent of degradation as undegraded, lightly degraded, moderately degraded, and severely
degraded to clarify the associations between microbial diversity (including bacteria and fungi) and potential soil
multifunctionality. Bacterial and fungal compositions were detected by sequencing of the 16S rDNA and internal
transcribed spacer amplicons, respectively. Functions associated with nutrient cycling (dissolved organic nitro-
gen and carbon, available phosphorus, NO 3
– , NH 4 + , C, N, P-cycle enzymes) and climate regulation (CO 2 and N 2 O
emissions) were also examined. Bacterial, rather than fungal, richness was negatively associated with potential
soil multifunctionality, which decreased along the degradation gradient. Structural equation modeling explained
79.6% of the variation in potential soil multifunctionality and confirmed that, in addition to bacterial community
richness and composition, organic carbon and moisture were important drivers of potential soil multi-
functionality. These results suggest that higher bacterial richness is associated with lower potential soil multi-
functionality in alpine meadow ecosystems. Among the bacterial taxa, only ~ 12% of bacterial genera were
identified as predictors of multifunctionality, suggesting functional redundancy of the bacterial community in the
meadow ecosystem. Rhodanobacter, Mucilaginibacter, Rhodococcus, and Bosea, belonging to the Proteobacteria
and the Actinobacteria phyla were identified as critical for maintaining potential soil multifunctionality. Our
results suggest that bacterial richness is negatively related to potential soil multifunctionality in alpine meadow
ecosystems and there is no correlation between potential multifunctionality and fungal richness.