Based on the long-term positioning experiment of sloping farmland ecosystem (1995-2019),bare
land (LD),control (CK,no fertilization),single application of nitrogen fertilizer (N1,N2: urea 55.2 kg·hm
-2
and 110.4 kg·hm
-2
),and combined application of nitrogen and phosphorus (N1P,N2P: urea + superphosphate
55.2+90 kg·hm
-2
and 110.4+90 kg·hm
-2
) of 6 treatments were designed to research the effect of long-term ferti-
lization on soil quality and millet yield. The results showed that: (1) Compared with CK,the long-term single ap-
plication of nitrogen fertilizer increased the soil alkaline nitrogen content by 24.81%~39.92%. The long-term com-
bined application of nitrogen and phosphorus fertilizers increased the soil organic carbon,total phosphorus,alkaline
nitrogen,and available phosphorus content by 7.82%~16.81%,58.18%,21.59%~22.89%,and 450.5%~660.3%,
and the soil pH decreased by 0.04~0.08 units. (2) Compared with CK,long-term single application of nitrogen
fertilizer alkaline phosphatase (AP) enzyme activity increased by 13.34%~19.43%. In the long-term nitrogen andphosphorus balanced fertilization soil,the enzyme activities of β -1,4-N-acetylglucosaminidase and leucine amin-
opeptidase (NAG+LAP),and glucosidase (βG) were significantly increased by 16.76% ~ 71.01%,47.12% ~
59.94%,respectively. Planting millet significantly increased the activities of βG,LAP+NAG,and AP by 68.74%
~342.42%. The stoichiometric ratio of enzyme in N1P treatment was closest to the steady state of 1 ∶ 1 ∶ 1.
(3) Compared with CK,the biomass and yield of millet under N1P treatment increased by 105.25% ~230.05%.
The yield of millet was significantly positively correlated with soil total phosphorus and available phosphorus,and
significantly negatively correlated with soil pH. In short,long-term fertilization significantly improved the soil
quality and millet yield of slope farmland in loess hilly areas,and the best outcome came from low nitrogen and
phosphorus combined application (N1P).
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