ISWC OpenIR  > 水保所知识产出(1956---)
长期施氮对谷子根系内生真菌群落特征的影响
高小峰1,2; 景航3; 闫本帅2; 吴春晓1,2; 王国梁1,3
2021
发表期刊水土保持学报
卷号35期号:5页码:303-311
摘要

为探讨长期施氮对作物运输根和吸收根内生真菌群落特征的影响及其机制。以农田长期定位施肥
试验( 1995 — 2019 年)为基础,通过高通量测序技术,研究长期 5 个施氮肥处理(
CK 、 N1 、 N2 、 N1P 、 N2P 。其
中 CK 、 N1 、 N2 施氮量分别为 0 , 55. 2 , 110. 4kg / hm
2 )对谷子不同功能根内生真菌群落组成和结构的影响。
结果表明: CK 处理中吸收根的 OTUs 数和真菌多样性(
shannon 指数和 Chao 1 指数)均显著高于运输根
( P <0.
05 )。门水平上,吸收根的 Ascomycota和 Glomeromycota丰度显著高于运输根( P <0. 05
),但 Mor

tierellomycota和 Basidiomycota丰度显著低于运输根( P <0.
05 )。在属水平上,优势菌属 Mortierella 和
Bipolaris 在吸收根内的相对丰度显著低于运输根( P <0. 05 ),但 Fusarium 和 Minimedusa 显著高于运输
根( P <0.
05 )。高氮施肥吸收根和运输根的 OTUs数、 Chao 1指数分别提高了23. 62% , 23. 87% ,其他施肥
则相反。施肥运输根的shannon指数提高了7. 12%~19. 62% ,吸收根则相反。施肥吸收根致病菌属( Bi -
polaris 、 Fusarium 、 Magnaporthiopsis 、
Microdochium )的相对丰度分别增加了52. 99% , 40. 74% , 133. 06% ,
200. 00% ,运输根中抗病菌属( Minimedusa )的相对丰度提高了 40. 16%~97. 11% 。施肥谷子根系碳、氮、磷
含量分别提高了 2. 33%~11. 63% , 13. 30%~94. 66% , 3. 89%~263. 92% ,氮磷肥配施处理的影响尤为显著
( P <0.
05 )。施肥运输根生物量提高了 106. 67%~336. 41% ,吸收根的生物量降低了 35. 20%~60. 20% 。
长期施肥通过提高土壤速效磷、全磷、可溶性氮和根系碳含量而提高吸收根内生真菌丰度,通过提高根系
生物量而降低运输根内生真菌丰度,低氮磷配施肥( N1P )对土壤养分含量及菌群发展影响最大。探明长期
施氮肥对黄土丘陵区谷子不同功能根系内生真菌分布格局变化的影响,为作物的高产优质及农田土壤生
态环境的良好构建提供理论依据。

其他摘要

 The purpose of this study was to explore the effect and its mechanism of long - term nitrogen appli -
cation on the characteristics of endophytic fungi community in crop transport roots and absorbing roots.
Based on the field long - term location fertilization experiment (
1995-2019 ), through the high - throughput se -
quencing technology , the long - term 5nitrogen fertilizer treatments (
CK , N1 , N2 , N1P , N2P ) were studied.
Among them , the CK , N1
, N2nitrogen application rates were respectively : 0 , 55.
2 , 110. 4kg / hm
2 )
on the
composition and structure of endophytic fungi community in different functional roots of millet.The results
showed that the number of OTUs and fungal diversity ( shannon index and Chao 1index ) of absorbing roots
in CK treatment were significantly higher than those of transporting
roots.At the phylum level , the abun -dance of Ascomycota and Glomeromycota of absorbing roots was significantly higher than that of transpor -
ting
roots , but the abundance of Mortierellomycota and Basidiomycota was significantly lower than that of
transporting roots.At the genus level
, the relative abundance of the dominant genus Mortierella and Bipo -
laris in absorbing roots was significantly lower than that in transporting roots , but Fusarium and Minime -
dusa were significantly higher than transporting roots.The number of OTUs and Chao 1index of absorbing
root and transport root of high nitrogen fertilization increased by 23.
62% , 23. 87% , respectively , while other
fertilization was just the opposite.The Shannon index of fertilization and transporting roots increased by
7. 12%~19. 62% , but the absorbing roots were the opposite.The relative abundance of fertilization - absorbing
root pathogenic bacteria ( Bipolaris , Fusarium , Magnaporthiopsis , Microdochium ) increased by 52. 99% ,
40. 74% , 133. 06% , 200. 00% , respectively , and the relative abundance of pathogen - resistant bacteria ( Min -
imedusa ) in transporting roots increased 40. 16%~97. 11%.The carbon , nitrogen , and phosphorus contents
of the millet root system increased by 2.
33%~11. 63% , 13. 30%~94. 66% , 3. 89%~263. 92% , respectively.
The effect of combined application of nitrogen and phosphorus was particularly significant.The biomass of
fertilization and transport roots increased by 106.
67%~336. 41% , and the biomass of absorbing roots was re -
duced by 35.
20%~60. 20%.Long - term fertilization increased the abundance of endophytic fungi in absorbing
roots by increasing
soil available phosphorus , total phosphorus , soluble nitrogen and root carbon content ,
and reducing the abundance of endophytic fungi in transporting
roots by increasing
root biomass , and apply -
ing phosphorus fertilizer with low nitrogen ( N1P ) has the greatest impact on the soil nutrient content and the
development of the flora.In short , long - term nitrogen application could change root endophytic flora by af -
fecting the soil or root chemical composition
, which might lead to the changes in plant survival strategies and
ultimately affect farmland ecosystems.

关键词长期施肥 氮磷 运输根 吸收根 内生真菌
收录类别中文核心期刊要目总览
语种中文
文献类型期刊论文
条目标识符sbir.nwafu.edu.cn/handle/361005/10411
专题水保所知识产出(1956---)
作者单位1.中国科学院水利部水土保持研究所黄土高原土壤侵蚀与旱地农业国家重点实验室
2.中国科学院大学
3.西北农林科技大学水土保持研究所
第一作者单位中国科学院水利部水土保持研究所
推荐引用方式
GB/T 7714
高小峰,景航,闫本帅,等. 长期施氮对谷子根系内生真菌群落特征的影响[J]. 水土保持学报,2021,35(5):303-311.
APA 高小峰,景航,闫本帅,吴春晓,&王国梁.(2021).长期施氮对谷子根系内生真菌群落特征的影响.水土保持学报,35(5),303-311.
MLA 高小峰,et al."长期施氮对谷子根系内生真菌群落特征的影响".水土保持学报 35.5(2021):303-311.
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